Composite

Part:BBa_K311002:Design

Designed by: Ethan Johnson, Claudia Schmidt-Dannert, Poonam Srivastava, Ian Windsor   Group: iGEM10_Minnesota   (2010-10-15)


Constitutive lac promoter with downstream EGFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 86
    Illegal XbaI site found at 104
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 86
    Illegal NotI site found at 988
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 86
    Illegal BglII site found at 243
    Illegal BamHI site found at 249
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 86
    Illegal XbaI site found at 104
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 86
    Illegal XbaI site found at 104
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The lac and EGFP were obtained from one of our home made plasmids by digesting it with EcoR I and Pst I. The part was ligated to EcoR I and Pst I cut pSB1C3.


Source

genomic DNA

References